Journal: ACS Omega

Article Title: Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis

doi: 10.1021/acsomega.1c07270

Figure Lengend Snippet: Relative mRNA expression rates of genes in chondrocytes by PCR. (A) SELE. (B) MMP2. (C) COL1. * P < 0.05, ** P < 0.01, *** P < 0.001 versus the IL-1β group.

Article Snippet: The following antibodies and corresponding dilution ratios were used: anti-SELE (bs-1273R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-MMP2 (bs-0412R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-CoL1 (bs-10423R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), and anti-GAPDH (60004-1-lg, Mouse, Proteintech, 120,000, secondary antibody, 1:5000).

Techniques: Expressing

Journal: ACS Omega

Article Title: Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis

doi: 10.1021/acsomega.1c07270

Figure Lengend Snippet: Western blot in five groups of chondrocytes. (A) SELE. (B) MMP2. (C) COL1. (D) Electrophoretic images. ** P < 0.01, *** P < 0.001 versus IL-1β group.

Article Snippet: The following antibodies and corresponding dilution ratios were used: anti-SELE (bs-1273R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-MMP2 (bs-0412R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-CoL1 (bs-10423R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), and anti-GAPDH (60004-1-lg, Mouse, Proteintech, 120,000, secondary antibody, 1:5000).

Techniques: Western Blot

Journal: ACS Omega

Article Title: Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis

doi: 10.1021/acsomega.1c07270

Figure Lengend Snippet: KEGG Enrichment Analysis

Article Snippet: The following antibodies and corresponding dilution ratios were used: anti-SELE (bs-1273R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-MMP2 (bs-0412R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-CoL1 (bs-10423R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), and anti-GAPDH (60004-1-lg, Mouse, Proteintech, 120,000, secondary antibody, 1:5000).

Techniques:

Journal: ACS Omega

Article Title: Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis

doi: 10.1021/acsomega.1c07270

Figure Lengend Snippet: PCR Primer Sequences

Article Snippet: The following antibodies and corresponding dilution ratios were used: anti-SELE (bs-1273R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-MMP2 (bs-0412R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), anti-CoL1 (bs-10423R, Rabbit, Bioss, 1:500, secondary antibody: 1:5000), and anti-GAPDH (60004-1-lg, Mouse, Proteintech, 120,000, secondary antibody, 1:5000).

Techniques: Sequencing

Journal: Molecular Medicine Reports

Article Title: Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway

doi: 10.3892/mmr.2015.4049

Figure Lengend Snippet: Girdin silencing inhibits the expression and activity of MMP-2 and MMP-9. (A and B) Changes in the mRNA levels of MMP-2 and MMP-9 were measured using reverse transcription-quantitative polymerase chain reaction following transfection. The relative mRNA expression levels were calculated using the 2 −ΔΔCt method. (C and D) Following transfection, changes in the protein levels of MMP-2 and MMP-9 were detected using western blot analysis. (E and F) Following transfection, gelatin zymography was performed to detect changes in the activities of MMP-2 and MMP-9. Each experiment was repeated three times. The experimental results are presented as the mean ± standard deviation. ** P<0.01, compared with the NC group. shRNA, short hairpin RNA; NC, negative control.

Article Snippet: Following washing with Tris-buffered saline with 0.05% Tween-20 (TBST), the membranes were incubated with the following primary antibodies at 4°C overnight: Rabbit anti-human polyclonal antibody against girdin (1:500 diluted; cat. no. bs-5150R; Bioss, Beijing, China); rabbit anti-human polyclonal antibody against MMP-2 (1:1,000 diluted; cat. no. WL0657); rabbit anti-human polyclonal antibody against MMP-9 (1:1,000 diluted; cat. no. WL0884); rabbit anti-human polyclonal antibody against P85α (1:1,000 diluted; cat. no. WL0191); rabbit anti-human polyclonal antibody against P110α (1:1,000 diluted; cat. no. WL0339); rabbit anti-human polyclonal antibody against AKT (1:1,000 diluted; cat. no. WL0003); rabbit anti-human polyclonal antibody against p-AKT (1:1,000 diluted; cat. no. WLP001).

Techniques: Expressing, Activity Assay, Real-time Polymerase Chain Reaction, Transfection, Western Blot, Zymography, Standard Deviation, shRNA, Negative Control

Journal: Stem Cells International

Article Title: Guanylate-Binding Protein 1 Promotes Migration and Invasion of Human Periodontal Ligament Stem Cells

doi: 10.1155/2018/6082956

Figure Lengend Snippet: Primer sequence.

Article Snippet: Antibodies used in this study includes rabbit polyclonal anti-human GBP1 antibody (Invitrogen) and rabbit polyclonal anti-human MMP2 antibody (Cell Signaling Technology).

Techniques: Sequencing

Journal: Stem Cells International

Article Title: Guanylate-Binding Protein 1 Promotes Migration and Invasion of Human Periodontal Ligament Stem Cells

doi: 10.1155/2018/6082956

Figure Lengend Snippet: GBP1 is required for upregulation of MMP2 by IFN- γ treatment in PDLSCs. RT-qPCR analysis of matrix metalloproteinase gene expression after 48 hours of IFN- γ treatment in PDLSCs and control cells (a–d). MMP2 expression was upregulated by IFN- γ treatment in a dose-dependent manner (b and e). The IFN- γ -induced upregulation of MMP2 was inhibited by depletion of GBP1 as determined by RT-qPCR analysis and Western blot (f and g). ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Antibodies used in this study includes rabbit polyclonal anti-human GBP1 antibody (Invitrogen) and rabbit polyclonal anti-human MMP2 antibody (Cell Signaling Technology).

Techniques: Quantitative RT-PCR, Gene Expression, Control, Expressing, Western Blot